anti human mouse plk1 antibody  (Bioss)

Journal: Pharmaceutics

Article Title: Sulfonated Amphiphilic Poly(α)glutamate Amine—A Potential siRNA Nanocarrier for the Treatment of Both Chemo-Sensitive and Chemo-Resistant Glioblastoma Tumors

doi: 10.3390/pharmaceutics13122199

Figure Lengend Snippet: The development of resistance to TMZ enhances MGMT expression without altering Plk1 oncogene levels in GB. ( A ) Survival analysis performed on OSgbm database comparing MGMT expression (left panel) and Plk1 expression (right panel) of the top 25% longest GB survivors versus the bottom 25% shortest survivors. ( B ) Inhibitory concentration 50% (IC 50 ) plot of parental U251 glioblastoma cells vs. TMZR clone. ( C ) Table summarizing the IC 50 of wild-type U251 cells versus U251 TMZ-R cells. ( D ) TMZ resistance in U251 occurred via upregulation of MGMT, without changing Plk1 levels. ( E ) Intracranial U251 GB tumors show Plk1 expression (scale = 100 µm).

Article Snippet: Cryo-sections were immunostained for: Plk1- using rabbit anti-human/mouse Plk1 antibody (Cat. No. BS-3535R dilution 1:50, Bioss Antibodies, Woburn, MA, USA) and Alexa Fluor 488-goat anti-rabbit secondary antibody (Cat. No. ab150077, dilution 1:300, Abcam, Cambridge, UK); SELP- using mouse anti-human SELP antibody (Cat. No. BBA1, Clone BBIG-E, dilution 1:30, R&D systems, McKinley, MN, USA) and Alexa Fluor 568-goat anti-mouse secondary antibody (Cat. No. ab175473, dilution 1:300, Abcam, Cambridge, UK).

Techniques: Expressing, Concentration Assay

Journal: Pharmaceutics

Article Title: Sulfonated Amphiphilic Poly(α)glutamate Amine—A Potential siRNA Nanocarrier for the Treatment of Both Chemo-Sensitive and Chemo-Resistant Glioblastoma Tumors

doi: 10.3390/pharmaceutics13122199

Figure Lengend Snippet: SELP expression on U251 and U251 TMZ-R 3D spheroids. ( A ) Imaged by confocal microscopy (scale = 20 µm) and ( B ) analyzed by fluorescence activated cell sorting (FACS). ( C ) SELP expression on slices of U251 intracranial tumors and co-localization with Plk1 (scale = 100 µm).

Article Snippet: Cryo-sections were immunostained for: Plk1- using rabbit anti-human/mouse Plk1 antibody (Cat. No. BS-3535R dilution 1:50, Bioss Antibodies, Woburn, MA, USA) and Alexa Fluor 488-goat anti-rabbit secondary antibody (Cat. No. ab150077, dilution 1:300, Abcam, Cambridge, UK); SELP- using mouse anti-human SELP antibody (Cat. No. BBA1, Clone BBIG-E, dilution 1:30, R&D systems, McKinley, MN, USA) and Alexa Fluor 568-goat anti-mouse secondary antibody (Cat. No. ab175473, dilution 1:300, Abcam, Cambridge, UK).

Techniques: Expressing, Confocal Microscopy, Fluorescence, FACS

Journal: Pharmaceutics

Article Title: Sulfonated Amphiphilic Poly(α)glutamate Amine—A Potential siRNA Nanocarrier for the Treatment of Both Chemo-Sensitive and Chemo-Resistant Glioblastoma Tumors

doi: 10.3390/pharmaceutics13122199

Figure Lengend Snippet: Sulfonate modification maintained size and activity of APA complexes. ( A ) Electrophoretic shift assay (EMSA) of polyplexes of APA and APAS with siRNA at increasing N/P ratios. ( B ) Table summarizing the hydrodynamic diameter, polydispersity index (PDI), and zeta potential of the main population of APAS:siPlk1 polyplexes as obtained by Mobius and PALS instruments. ( C ) Specific Plk1 mRNA silencing obtained by RT-PCR, performed on U251 and U251 TMZ-R GB cells following treatment with APAS:siPlk1 polyplexes. ( D , E ) Specific MGMT mRNA silencing obtained by RT-PCR ( D ) and western Blot ( E ) performed on U251 and U251 TMZ-R GB cells following treatment with APAS:siMGMT polyplexes.

Article Snippet: Cryo-sections were immunostained for: Plk1- using rabbit anti-human/mouse Plk1 antibody (Cat. No. BS-3535R dilution 1:50, Bioss Antibodies, Woburn, MA, USA) and Alexa Fluor 488-goat anti-rabbit secondary antibody (Cat. No. ab150077, dilution 1:300, Abcam, Cambridge, UK); SELP- using mouse anti-human SELP antibody (Cat. No. BBA1, Clone BBIG-E, dilution 1:30, R&D systems, McKinley, MN, USA) and Alexa Fluor 568-goat anti-mouse secondary antibody (Cat. No. ab175473, dilution 1:300, Abcam, Cambridge, UK).

Techniques: Modification, Activity Assay, Shift Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Polo-like kinase 1 is overexpressed in colorectal cancer and participates in the migration and invasion of colorectal cancer cells

doi: 10.12659/MSM.882900

Figure Lengend Snippet: Sequences of small synthetic oligonucleotides unique to PLK1.

Article Snippet: The slides were incubated with primary antibody diluted 1:50 in Tris-buffered saline pH 7.6 (TBS) with 1% BSA of mouse anti-human PLK1 (Santa Cruz, USA) for 2 hours at room temperature.

Techniques: Negative Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Polo-like kinase 1 is overexpressed in colorectal cancer and participates in the migration and invasion of colorectal cancer cells

doi: 10.12659/MSM.882900

Figure Lengend Snippet: PLK1 and PCNA expression in colorectal tissues, n (%).

Article Snippet: The slides were incubated with primary antibody diluted 1:50 in Tris-buffered saline pH 7.6 (TBS) with 1% BSA of mouse anti-human PLK1 (Santa Cruz, USA) for 2 hours at room temperature.

Techniques: Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Polo-like kinase 1 is overexpressed in colorectal cancer and participates in the migration and invasion of colorectal cancer cells

doi: 10.12659/MSM.882900

Figure Lengend Snippet: Expression of PLK1 and PCNA in colorectal tissues. ( A ) PLK1 negatively expressed in normal colorectal tissues; ( B ) PLK1 moderately expressed in colorectal cancer tissues; ( C ) PLK1 strongly expressed in colorectal cancer tissues; ( D ) PCNA negatively expressed in normal colorectal tissues; ( E ) PCNA moderately expressed in colorectal cancer tissues; ( F ) PCNA strongly expressed in colorectal cancer tissues. Original magnifications ×200.

Article Snippet: The slides were incubated with primary antibody diluted 1:50 in Tris-buffered saline pH 7.6 (TBS) with 1% BSA of mouse anti-human PLK1 (Santa Cruz, USA) for 2 hours at room temperature.

Techniques: Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Polo-like kinase 1 is overexpressed in colorectal cancer and participates in the migration and invasion of colorectal cancer cells

doi: 10.12659/MSM.882900

Figure Lengend Snippet: Association between PLK1 expression and clinical characteristics of colorectal cancers, n (%).

Article Snippet: The slides were incubated with primary antibody diluted 1:50 in Tris-buffered saline pH 7.6 (TBS) with 1% BSA of mouse anti-human PLK1 (Santa Cruz, USA) for 2 hours at room temperature.

Techniques: Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Polo-like kinase 1 is overexpressed in colorectal cancer and participates in the migration and invasion of colorectal cancer cells

doi: 10.12659/MSM.882900

Figure Lengend Snippet: Correlation between expression of PLK1 and PCNA in colorectal cancer.

Article Snippet: The slides were incubated with primary antibody diluted 1:50 in Tris-buffered saline pH 7.6 (TBS) with 1% BSA of mouse anti-human PLK1 (Santa Cruz, USA) for 2 hours at room temperature.

Techniques: Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Polo-like kinase 1 is overexpressed in colorectal cancer and participates in the migration and invasion of colorectal cancer cells

doi: 10.12659/MSM.882900

Figure Lengend Snippet: Expression and inhibition of PLK1 in colorectal cancer cells lines. ( A ) PLK1 mRNA expression detected in 9 colorectal cancer cell lines by PCR; ( B ) PLK1 protein expression detected in 9 colorectal cancer cell lines by Western blotting; ( C ) PLK1 mRNA expression detected in siRNA-treated SW1116 by real-time PCR at 24 hours after transfection. Values are mean ±SD of the expression amount of PLK1 relative to GAPDH; (D) PLK1 protein expression detected in siRNA-treated SW1116 by Western blotting at 48 hours and 72 hours after transfection.

Article Snippet: The slides were incubated with primary antibody diluted 1:50 in Tris-buffered saline pH 7.6 (TBS) with 1% BSA of mouse anti-human PLK1 (Santa Cruz, USA) for 2 hours at room temperature.

Techniques: Expressing, Inhibition, Western Blot, Real-time Polymerase Chain Reaction, Transfection

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Polo-like kinase 1 is overexpressed in colorectal cancer and participates in the migration and invasion of colorectal cancer cells

doi: 10.12659/MSM.882900

Figure Lengend Snippet: Effect of PLK1 depletion on migration and invasion of SW1116 cells. ( A ) 6.5 mm Transwell® with 8 μm pore Polycarbonate Membrane Insert were used for migration and invasion (with Matrigel) assay. The chambers were stained with methyl violet (×4 for migration, ×10 for invasion). ( B ) The migration and invasion cells were counted in 5 random fields of vision and the number of cells is expressed as mean ±SD for 3 replication experiments.

Article Snippet: The slides were incubated with primary antibody diluted 1:50 in Tris-buffered saline pH 7.6 (TBS) with 1% BSA of mouse anti-human PLK1 (Santa Cruz, USA) for 2 hours at room temperature.

Techniques: Migration, Matrigel Assay, Staining

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Polo-like kinase 1 is overexpressed in colorectal cancer and participates in the migration and invasion of colorectal cancer cells

doi: 10.12659/MSM.882900

Figure Lengend Snippet: Effect of PLK1 depletion on cells proliferation and apoptosis. ( A ) CCK-8 was used for determining the role of PLK1 in regulating SW1116 proliferation at different moments. Values are the mean ±SD of absorbance at 450 nm for 6 replication experiments. ( B,C) FCM was used to determine the role of PLK1 in regulating apoptosis of SW1116 at different moments. Annexin V and PI stained cells were detected by FCM. Values are the mean ±SD of apoptosis rate (including early and late apoptosis) for the 3 replication experiments.

Article Snippet: The slides were incubated with primary antibody diluted 1:50 in Tris-buffered saline pH 7.6 (TBS) with 1% BSA of mouse anti-human PLK1 (Santa Cruz, USA) for 2 hours at room temperature.

Techniques: CCK-8 Assay, Staining

Journal: Frontiers in Oncology

Article Title: A novel association of pyroptosis-related gene signature with the prognosis of hepatocellular carcinoma

doi: 10.3389/fonc.2022.986827

Figure Lengend Snippet: Pyroptosis-related Gene Signature with the Prognosis of Hepato cellular Carcinoma.

Article Snippet: The primary antibodies used for IHC staining were rabbit anti-human CHMP4A (Cat# PA5-117867, Invitrogen, 1:100), mouse anti- human HMGB1 (Cat#ab77302, Abcam, 1:200) and mouse anti-human PLK1 (Cat#37-7000, Invitrogen, 1:100).

Techniques: Binding Assay

Journal: Frontiers in Oncology

Article Title: A novel association of pyroptosis-related gene signature with the prognosis of hepatocellular carcinoma

doi: 10.3389/fonc.2022.986827

Figure Lengend Snippet: Primer name and primer sequence.

Article Snippet: The primary antibodies used for IHC staining were rabbit anti-human CHMP4A (Cat# PA5-117867, Invitrogen, 1:100), mouse anti- human HMGB1 (Cat#ab77302, Abcam, 1:200) and mouse anti-human PLK1 (Cat#37-7000, Invitrogen, 1:100).

Techniques: Sequencing

Journal: Frontiers in Oncology

Article Title: A novel association of pyroptosis-related gene signature with the prognosis of hepatocellular carcinoma

doi: 10.3389/fonc.2022.986827

Figure Lengend Snippet: Validation of the differential expression of the three prognostic genes in clinical samples. (A) The mRNA levels of CHMP4A , HMGB1 and PLK1 were detected in normal tissues and HCC tissues from the GEPIA * P < 0.05. (B) Immunohistochemistry of the CHMP4A, HMGB1 and PLK1 was determined in HCC tissues and normal tissues from the HPA database.

Article Snippet: The primary antibodies used for IHC staining were rabbit anti-human CHMP4A (Cat# PA5-117867, Invitrogen, 1:100), mouse anti- human HMGB1 (Cat#ab77302, Abcam, 1:200) and mouse anti-human PLK1 (Cat#37-7000, Invitrogen, 1:100).

Techniques: Expressing, Immunohistochemistry

Journal: Frontiers in Oncology

Article Title: A novel association of pyroptosis-related gene signature with the prognosis of hepatocellular carcinoma

doi: 10.3389/fonc.2022.986827

Figure Lengend Snippet: Validation of the differential expression of the three prognostic genes in vivo and in vitro . (A) Typical diagrams of normal and tumor liver tissue from mice were presented. (B) Tumor volumes between normal and HCC models from mice were measured. (n = 5, per group) (C) The mRNA levels of CHMP4A , HMGB1 and PLK1 were detected in the normal and HCC livers of mice. (n = 5, per group) (D) The mRNA levels of CHMP4A , HMGB1 and PLK1 were assessed between HL-7702 and Huh7 cells. (n = 3) * P < 0.05; *** P < 0.001. (E) The expression of CHMP4A, HMGB1 and PLK1 was determined in HCC tissues and non-tumor tissues from human samples performed by immunohistochemistry.

Article Snippet: The primary antibodies used for IHC staining were rabbit anti-human CHMP4A (Cat# PA5-117867, Invitrogen, 1:100), mouse anti- human HMGB1 (Cat#ab77302, Abcam, 1:200) and mouse anti-human PLK1 (Cat#37-7000, Invitrogen, 1:100).

Techniques: Expressing, In Vivo, In Vitro, Immunohistochemistry

Journal: World Journal of Gastroenterology : WJG

Article Title: Polo-like kinase 1, a new therapeutic target in hepatocellular carcinoma

doi: 10.3748/wjg.v18.i27.3527

Figure Lengend Snippet: Table 1

Article Snippet: After blocking, the membrane was incubated with mouse anti-human Plk1 (F-8) antibody (Santa Cruz Biotechnology, Santa Cruz, CA, United States) at 1:500 for 1 h at 22 °C followed by incubation with Cruz Marker Compatible goat anti-mouse IgG antibody (Santa Cruz) at 1:50 000 for 1 h at 22 °C.

Techniques: Gene Expression